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EXPERIMENTAL PROCEDURES A. Preparation of SAMs by Zwitterion Dopamine Molecule A metallic substrate was prepared by deposition of titanium onto a 20 mm × 20 mm glass slide in a high-vacuum thermal evaporator. A 50-nm thick layer of titanium was © Springer International Publishing Switzerland 2015 I. 1007/978-3-319-11128-5_8 24 Novel Bio-inspired Zwitterion Dopamine Molecule for Anti-biofouling and Photocleavable Properties formed and oxidized in ambient to have a layer of oxide. The TiO2 substrates were cleaned by thoroughly washing with 1% SDS detergent, deionized water, and absolute ethanol, followed by drying in a stream of nitrogen.

Springer International Publishing Switzerland 2015 I. -H. Chu et al. In this study, X-ray photoelectron spectroscopy (XPS) was utilized to identify the elemental composition of SAMs. The surface wetting was measured using a contact angle goniometer. For the bacterial adsorption, gram positive (Staphylococcus epidermidis) and negative (Pseudomonas aeruginosa), the common bacterial pathogens of humans, was used to test the antifouling properties of amino acids SAMs surface. MTT cytotoxicity assays were performed to evaluate biocompatibility by 3T3 cells cell viability.

2009;4:FA3-FA16. Vasita R, Shanmugam K, Katti DS. Improved biomaterials for tissue engineering applications: Surface modification of polymers. Curr Top Med Chem. 2008;8:341-53. Chen SF, Li LY, Zhao C, Zheng J. Surface hydration: Principles and applications toward low-fouling/nonfouling biomaterials. Polymer. 2010;51:5283-93 Love JC, Estroff LA, Kriebel JK, Nuzzo RG, Whitesides GM. Self-assembled monolayers of thiolates on metals as a form of nanotechnology. Chem Rev. 2005;105:1103-69. Kondoh H, Kodama C, Nozoye H.

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