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Rev. 14, 72–93. 52. , and Langin, D. (1995) Molecular mechanisms underlying regional variations of catecholamine-induced lipolysis in rat adipocytes. Am. J. Physiol. 268, E1135–E1142. 53. Bartness, T. , Hamilton, J. , Wade, G. , and Goldman, B. D. (1989) Regional differences in fat pad responses to short days in Siberian hamsters. Am. J. Physiol. 257, R1533–R1540. 54. , and Pi-Sunyer X. (1982) Variations in glucose metabolism by fat cells from three adipose depots of the rat. Metabolism 31, 876–883.

44 Cinti et al. 2. IBAT is cut into two parts along the middle plane of symmetry; each hemi-IBAT is paraffin-embedded and sectioned along parasagittal planes; 3-µm-thick serial sections are collected every millimeter and stained with H&E. 3. For each section, connective-vascular, unilocular, and multilocular areas are identified with a camera lucida, and measured with the Kontron image analyzer (45). 4. For each animal, 500 profiles of multilocular cells are measured to determine mean cell volume.

Sci. 11, 1099–1106. 79. , Koza, R. , and Kozak, L. P. (1998) Emergence of brown adipocytes in white fat in mice is under genetic control: effect on body weight and adiposity. J. Clin. Invest. 102, 412–420. Choosing an AT Depot 19 80. , et al. (1995) Adrenergic receptors and fat cells: differential recruitment by physiological amines and homologous regulation. Obesity Res. 3, 507S–514S. 81. , and Nougues, J. (1998) In vitro induction of UCP1 mRNA in preadipocytes from rabbit considered as a model of large mammals brown adipose tissue development: importance of PPARgamma agonists for cells isolated in the postnatal period.

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