By Weiming Xia, Huaxi Xu

Within the look for an efficient therapy for Alzheimer's affliction, APP is a distinct version protein that illustrates the big choice of simple and complex characterization recommendations on hand.

Exploring various organic suggestions to explain the constitution and serve as of this transmembrane protein, this article offers every one process with designated, step by step protocols to accomplish reproducible effects and supply a framework for learning different membrane proteins.

Show description

Read or Download Amyloid precursor protein : a practical approach PDF

Similar basic science books

Plasma Lipids and Their Role in Disease (Advances in Vascular Biology)

This quantity offers up to date info on plasma lipid shipping, some of the different types of plasma lipid issues and the connection among plasma lipids, lipoproteins, and atherosclerotic sickness.

Human Mitochondrial DNA and the Evolution of Homo sapiens (Nucleic Acids and Molecular Biology, 18)

Mitochondrial DNA is without doubt one of the such a lot heavily explored genetic structures, since it can let us know loads concerning the human earlier. This ebook takes a different standpoint, featuring the disparate strands that needs to be tied jointly to take advantage of the program. From molecular biology to anthropology, information to historic DNA, this primary quantity of 3 provides a finished international photo and a serious appraisal of human mitochondrial DNA edition.

Handbook of Human Stress and Immunity

In 1964, George Solomon coined the time period psychoneuroimmunology. within the intervening 30 years, this time period has emerged right into a dynamic box of analysis which investigates the original interactions among the worried, endocrine, and immune platforms. The guide of Human rigidity and Immunity is a entire reference for this dynamic new box.

Pocket Podiatry: Functional Anatomy

Pocket Podiatry: useful Anatomy promises all of the necessities of exam and prognosis in a handy, undemanding structure. With the emphasis on sensible, step by step information, this convenient quantity is particularly designed to incorporate priceless diagrams, tables, counsel and precis packing containers to offer you easy access to key details with the minimal of fuss.

Additional info for Amyloid precursor protein : a practical approach

Sample text

The fourth and final is a morphological approach to examine APP internalization. 1,22 The 1G7 antibody is radioiodinated using IODO-GEN precoated iodination tubes (Pierce, Rockford, IL; #28601). fm Page 22 Wednesday, November 3, 2004 2:59 PM to the antibody. Excessive iodine is finally removed by gel filtration, typically with a disposable NAP-5 (Sephadex G-25) column (Amersham Biosciences, Piscataway, NJ; #17-0853-01). The entire procedure must be performed in an approved protective hood to avoid uptake of free radioactive iodine.

5). Sequence alignments of APP orthologues and paralogues show their GFD regions are well conserved with sequence identities ranging from 36 to 84%; all the cysteine residues are strictly conserved. This suggests that the fold described here is maintained across the APP family. 19 Maintenance of the disulfide bridge in this region is critical for neurite outgrowth19 and activation of MAP kinase,27 suggesting that the conformation of the loop is important. The surface is dominated by the β-hairpin loop (residues 96 to 110) representing the most mobile region of the structure.

Cell Sci. 109, 991, 1996. 20. Soriano, S. et al. Expression of beta-amyloid precursor protein-CD3gamma chimeras to demonstrate the selective generation of amyloid beta(1-40) and amyloid beta(142) peptides within secretory and endocytic compartments. J. Biol. Chem. 274, 32295, 1999. 21. U. et al. The cytoplasmic domain of the LDL receptor-related protein regulates multiple steps in APP processing. EMBO J. 21, 5691, 2002. 22. H. Trafficking of cell surface beta-amyloid precursor protein: retrograde and transcytotic transport in cultured neurons.

Download PDF sample

Rated 4.38 of 5 – based on 15 votes